DEVELOPMENT OF A NEW COTTON WASTE COMPOSTING TECHNOLOGY FOR CULTIVATION OF OYSTER MUSHROOM (Pleurotus ostreatus)

This applicable research has successful selected enzyme Ctec2 from Novozyme (Denmark) which gave the best cellulose degradation activity compared to the other enzymes. At optimum conditions of 50 o C, pH=5 and ratio between enzyme and substrate (E/S) being 0.40%, 0.45%, 0.50%, the time needed to substrate degrade was 60 hours. Based on this finding, a new cotton waste composting technology for cultivating oyster mushroom was developed. Mushroom productivities using the new technology had reached 62.3%, 65.1%, 59.8% respectively, over dry weight of the substrate. The application of this technology can help to reduce the substrate processing time from 06 days to 4.5 days and the fiber phase was finished 7-10 days earlier. The mushroom yield found 65.1% increase over the dry weight of the substrate and was 20% higher than using the natural fermentation method.